The active fraction or fraction containing the active compound will be tested for insulin secretion using pancreatic beta cells in vitro. The active extract will be fractionated using various chromatographic methods and the pure compound(s) will be characterized using spectroscopic and chemical methods. The active fractions will be purified using bioassay-guided fractionation to obtain pure bioactive components. The extracts, free from solvent, will be assayed for insulin secretion. The pit-free fruits will be extracted first with water and the residue extracted sequentially with methanol and ethylacetate. Fresh fruits will also be extracted if available and in that case, frozen fruits will not be used. The fruits will be harvested when ripe and stored at -20 C until extraction. Project Methods The proposed research will focus on Cornus mas fruits. mas extracts/compounds to stimulate acute insulin release (B) whether long-term consumption of Cornus extracts/compounds can potentiate insulin release and lower hyperglycemia in a mouse model of type II diabetes mellitus. mas extracts and purified compounds in diabetic mouse C57BL/KsJ for (A) the ability of oral consumption of C. masįruits, (II) To conduct insulin secretion assay using pancreatic beta cells in vitro, (III) To evaluate the in vivo efficacies of C. Therefore, the specific objectives of our proposal are as follows: (I) To conduct bioassay-directed isolation and characterization of active components that show antidiabetic activity in Michigan grown C. mas as an economical and plentiful source of human health products and to develop purification and separation processes for the production of nutraceuticals. mas extract from fruits collected in Michigan induced insulin secretion in insulin assay using INS-cells, in addition to being a strong antioxidant. In our preliminary bioactivity studies, C. Glycosides of cyanidin, pelargonidin and delphinidin aglycones have been previously reported from the fruits. A literature survey of the chemical investigation of Cornus mas shows that attention has been confined to only the anthocyanins present in the fruits. is known as Cornelian cherry and its dark red berries are consumed in various parts of Europe in the form of a jam. The proposed research is expected to demonstrate health effects derived from the consumption of specific components from Cornelian cherry fruits and expected to lead to a greater understanding of the potential benefits of Cornelian cherry products to human health. If successful this research will lead to consumption of Cornelian cherry by diabetic patients, increased nursery production of Cornelian cherry, provide an alternative horticultural crop forĬurrent producers, and spawn a new industry to develop value-added Cornelian cherry fruit-products in the form of phytomedicines and human health products to prevent diabetes. Therefore, our approach will be to conduct bioassay-guided fractionation and purification of components that are capable of producing insulin in Cornelian cherry fruits and determine the insulin production by these compounds in mouse models. Most type-2 diabetes patients supplement insulin by daily injections. population and 18.4% of those over age 65. Type-2 diabetes affects almost six percent of the U.S. Diabetes is the seventh leading killer in the U.S.A. mas extract from fruits collected in Michigan induced insulin secretion in mouse pancreatic beta cell culture, in addition to being a strong antioxidant.
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